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DAT Biology Questions: 6 Free Practice Tests 2023

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Are you looking for a quick way to practice what you have learned and prepare for DAT biology section?

Well, you came to the right place.

We have been working for the last several months on creating high-yield DAT Biology questions that can help anyone preparing for the DAT Biology test.

These are free DAT practice tests, no tricks or anything like that.

We want to help pre-dental students prepare for the DAT and what a better way than actually using some exam-like practice questions to test your knowledge of Biology.

By taking these DAT practice tests, you can identify areas where you need to improve, strengthen your knowledge base, and ultimately boost your DAT Biology score.

You will find below 6 DAT biology practice tests with each test having a total of 40 biology questions. The idea here is to simulate what you will be tested on when you take your DAT test.

As always, feel free to leave any feedback you have about these questions. Our goal is to improve these tests and provide more free similar practice tests.

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DAT Biology Practice Test #1

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1. The innate behavior where an animal responds to a stimulus without thought is known as:

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2. What is the euphotic zone in aquatic biomes?

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3. What does it mean for a species to be invasive?

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4. How do vestigial structures provide evidence for evolution?

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5. Which of the following best defines evolution?

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6. Which of the following reproductive methods involves offspring developing and hatching outside of the mother's body?

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7. In which layer of the epidermis do keratinocyte stem cells proliferate?

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8. Where are eccrine glands located?

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9. Which substances are almost completely reabsorbed by the nephron due to their importance in the body?

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10. What best describes the relationship between marine fish and their environment in terms of osmoregulation?

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11. What is the role of enteropeptidase in digestion?

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12. What is the function of Antidiuretic hormone (ADH)?

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13. What type of feedback loop promotes stability in the body by preventing hormone overproduction?

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14. Which of the following types of cartilage is highly flexible and found in ears and epiglottis?

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15. What is the function of the epiphysis in a long bone?

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16. What is the deepest sheath that covers the muscle fibers?

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17. What happens when a stimulus causes the threshold potential to be reached in a neuron?

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18. What is the function of the myelin sheath?

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19. Which type of presentation involves T cells binding to antigens presented on APCs and then releasing interleukins to boost both innate immunity and adaptive immunity?

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20. Which of the following is an example of external immunity?

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21. What is the main intracellular buffer system in humans?

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22. The sinoatrial (SA) node is responsible for initiating the cardiac cycle. Which of the following statements is true about it?

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23. What is the correct formula for calculating cardiac output (CO)?

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24. What is a characteristic feature of bryophytes?

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25. What is the first part of the embryo to emerge during the process of germination?

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26. What is the primary mode of reproduction for organisms in the Platyhelminthes phylum?

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27. How are DNA microarrays used in genomics research?

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28. What is the correct order of organelles from most dense to least dense in density centrifugation?

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29. What is an example of polygenic inheritance?

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30. What is the definition of heredity?

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31. In the structure of nucleosomes, which histone holds the DNA in place?

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32. What are the sister chromatids in a dyad connected by?

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33. What is the function of the pericentriolar material?

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34. What occurs during the process of non-cyclic photophosphorylation?

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35. Which cells in the leaf structure are responsible for most of the photosynthesis?

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36. What is the purpose of the electron transport chain in oxidative phosphorylation?

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37. What is the main difference between the structures of eukaryotic and prokaryotic flagella?

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38. What is the main difference between an agonist and an antagonist in cell signaling?

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39. Which of the following is a exception to the central dogma of genetics?

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40. Which of the following is not a component of carbohydrates?

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DAT Biology Practice Test #2

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1. What is the term for the behavior used to protect an animal's territory or safe space?

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2. Who are the primary consumers in an ecosystem?

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3. What role do decomposers play in an ecosystem?

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4. What does "anagenesis" describe in evolutionary biology?

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5. According to the theory of punctuated equilibrium, how does evolution occur?

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6. What is the function of the notochord during neurulation?

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7. Which type of gland is not found in the palms of hands and soles of feet?

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8. What is the function of the hypodermis?

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9. Which of the following best describes the role of renin in the Renin Angiotensin Aldosterone System (RAAS)?

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10. Where does filtration primarily occur in the nephron?

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11. How does the liver handle the dangerous byproduct of protein metabolism, ammonia?

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12. In females, which hormone during the menstrual cycle causes ovulation?

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13. The hormone FSH in males stimulates what?

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14. Which of the following hormones increases blood calcium levels by stimulating osteoclasts and depressing osteoblasts?

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15. What is the function of the epiphyseal plate?

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16. Which of the following statements accurately describes the I band?

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17. What type of cells form the myelin sheath in the peripheral nervous system?

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18. Which neurotransmitter is associated with the "fight or flight" response?

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19. Which type of immunity is responsible for the production and release of antibodies?

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20. Which type of leukocyte is responsible for the first part of the inflammatory response?

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21. Which of the following is NOT a component of the bicarbonate buffering system?

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22. What is the primary function of the umbilical vein in fetal circulation?

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23. Which of the following statements is true about blood clotting?

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24. What is the role of auxins in plant growth?

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25. Which type of growth occurs at the apical meristems and leads to vertical growth in plants?

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26. Which type of chordate lacks a heart in its circulatory system?

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27. What does the term "competent" mean in the context of bacterial cloning?

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28. What is the process of creating millions of copies of DNA called?

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29. Which term refers to a sequence of DNA that codes for a trait?

30 / 40

30. What is the difference between incomplete dominance and codominance?

31 / 40

31. What is the primary difference between the action of exonucleases and endonucleases on a polynucleotide chain?

32 / 40

32. What happens during the G0 phase of the cell cycle?

33 / 40

33. What happens in the prophase of mitosis?

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34. What is the main drawback in the process of C4 photosynthesis?

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35. In which part of the plant cell does the Calvin cycle take place?

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36. In the Cori cycle, where does lactate get transported to be converted back into glucose?

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37. What is the term used to describe the process by which a cell's cytoplasm shrinks away from the cell wall due to water loss from being in a hypertonic solution?

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38. Which factor affects the fluidity of the cell membrane?

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39. What nitrogenous base is found in RNA instead of thymine?

40 / 40

40. What is the empirical formula of monosaccharides?

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DAT Biology Practice Test #3

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1. Which type of mating involves mating once in a lifetime and producing multiple offspring without parental care?

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2. Which of the following behaviors is most accurately classified as a Fixed Action Pattern?

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3. What happens to the energy stored in a trophic level as it is transferred to the next trophic level?

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4. What does a cladogram represent?

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5. Which of the following is not an example of prezygotic isolation?

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6. Regarding stem cells, which of the following is the correct sequence from most potent to least potent?

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7. Which of the following accurately describes binary fission?

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8. Which layer of the epidermis is important for strength and immunity?

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9. What is the primary function of Atrial natriuretic peptide (ANP) when released by atrial cells?

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10. What is the primary force driving filtration in Bowman’s capsule?

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11. What does the liver do when glycogen stores are depleted?

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12. Which of the following best describes the role of pseudopodia in amoebas?

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13. What does the hormone aldosterone function to do?

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14. Which of the following cell types is responsible for bone resorption during bone remodeling?

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15. What is the function of cancellous bone?

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16. Which of the following types of motor units is used for powerful movements?

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17. Which of the following statements is true regarding the sliding filament theory of muscle contraction?

18 / 40

18. Which neurotransmitter is associated with reward, pleasure, and addiction?

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19. What is the function of platelets in the innate immune system?

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20. Which type of leukocyte makes up over half of all leukocytes?

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21. Which of the following is NOT a reason for a left-shifted oxygen dissociation curve?

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22. Which of the following is true about respiration in Cnidaria?

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23. Which type of blood can receive blood from any donor?

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24. What is the function of the Casparian strip in the roots of plants?

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25. Which type of cell in the xylem allows for water transport through pits in their tapered ends?

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26. Which of the following chordates have a two-chambered heart?

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27. What is the primary distinguishing feature between prokaryotes and eukaryotes?

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28. What type of blotting is used to identify fragments of known RNA?

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29. What does the term "heterozygous" mean?

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30. What is the role of proto-oncogenes in cancer development?

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31. Which of the following is not a potential outcome of base substitutions (point mutations) in DNA?

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32. How does acetylation of histones affect gene transcription?

33 / 40

33. What distinguishes meiosis I from meiosis II?

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34. What is the primary function of RuBisCo in the process of photosynthesis?

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35. What is the main difference between C4 photosynthesis and CAM photosynthesis?

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36. Alcohol fermentation includes an extra step compared to lactic acid fermentation. What does this step involve?

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37. What is the key difference between catabolic and anabolic processes in an organism's metabolism?

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38. Which of the following best describes the role of the sodium-potassium pump in the cell?

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39. Which of the following statements is NOT part of modern cell theory?

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40. What is the difference between amylose and amylopectin?

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DAT Biology Practice Test #4

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1. Which term refers to a behavior where one animal's threat causes another animal's submission, thus preventing aggression?

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2. A behavioral response that decreases in response to a repetitive, meaningless stimulus is known as:

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3. What is the principle of Malthus's population theory?

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4. What is the result of sympatric speciation?

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5. What is a 'polytomy' in the context of a phylogenetic tree?

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6. What is the role of the mesoderm layer in a trilaminar embryo?

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7. Which hormone is responsible for the maturation of sperm and the development of male secondary sex characteristics?

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8. What are the two layers of the dermis?

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9. What occurs as the filtrate travels down the descending limb of the loop of Henle?

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10. How does the length of the nephron affect the concentration of urine?

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11. Which hormone is responsible for slowing gastric emptying and stimulating the release of digestive enzymes and bile?

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12. What is the primary function of G cells in the stomach during digestion?

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13. Which of the following is the most common cause of goiter?

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14. Which type of bone is found within tendons to help muscles pull?

15 / 40

15. What is the function of Volkmann's canals?

16 / 40

16. Which of the following statements accurately describes the process of wave summation?

17 / 40

17. Which type of muscle is characterized by involuntary contractions?

18 / 40

18. Which part of the ear helps convert mechanical signals into neuronal signals?

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19. Which type of T cell is responsible for directly killing infected cells?

20 / 40

20. Which of the following is NOT a sign of inflammation?

21 / 40

21. Which of the following is true about carbon monoxide (CO) poisoning?

22 / 40

22. What is the difference between the left and right lungs in humans?

23 / 40

23. What is erythroblastosis fetalis?

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24. Which theory explains the upward movement of water in plants due to transpiration?

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25. What type of plant tissue provides protection and regulation and includes the epidermis and root hairs?

26 / 40

26. Which phylum is characterized by five-fold radial symmetry as adults?

27 / 40

27. What is the main difference between gram-positive and gram-negative bacteria?

28 / 40

28. What is the purpose of a genomic library?

29 / 40

29. What does gene linkage refer to?

30 / 40

30. What is the one-hit hypothesis associated with?

31 / 40

31. What is the purpose of the G1/S checkpoint in the cell cycle?

32 / 40

32. In which direction does RNA polymerase extend the RNA during transcription?

33 / 40

33. In terms of regulation, what is the role of cyclin-dependent kinases (CDKs) in the cell cycle?

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34. What is the primary difference between C4 and CAM photosynthesis?

35 / 40

35. What is the role of chlorophyll in the photosynthesis process?

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36. What function do High-density lipoproteins (HDLs) serve in the body?

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37. Why is ATP considered the cellular energy currency?

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38. What role does clathrin play in the transport of substances across the cell membrane?

39 / 40

39. Which of the following statements is true about catalysts?

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40. What is the definition of an element?

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DAT Biology Practice Test #5

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1. What does the concept of "inclusive fitness" refer to?

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2. In the context of animal behaviors, what does the term "stimulus discrimination" mean?

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3. Which of the following correctly represents the type II survivorship curve?

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4. What is the type of symbiosis where one organism benefits and the other organism is unaffected?

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5. In terms of genetic variation, what does "balanced polymorphism" refer to?

6 / 40

6. Which of the following correctly describes the process of capacitation in sperm cells?

7 / 40

7. In the context of birth control pills, how does the release of synthetic estrogen and progesterone prevent pregnancy?

8 / 40

8. What are the three possible shapes for epithelial cells?

9 / 40

9. What are the three layers of the integumentary system?

10 / 40

10. Which hormone is produced by the adrenal cortex and increases salt and water reabsorption and potassium secretion in the distal tubules and collecting ducts?

11 / 40

11. What is a key indicator of the health of the microbiome in the large intestine?

12 / 40

12. What is the primary function of bile in the digestive process?

13 / 40

13. Which of the following hormones is secreted by the parafollicular cells in the thyroid gland?

14 / 40

14. Which of the following hormones are derived from cholesterol?

15 / 40

15. Which of the following statements is true regarding fibrous connective tissue?

16 / 40

16. What structure quickens the propagation of action potentials on the muscle fiber?

17 / 40

17. What is the purpose of the sarcoplasmic reticulum in muscle fibers?

18 / 40

18. Which of the following is NOT a component of the limbic system?

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19. Which glial cell type produces cerebrospinal fluid?

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20. What is the function of memory B cells?

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21. Which of the following is true about myoglobin?

22 / 40

22. What is the minimum amount of air that needs to be present in the lungs to prevent collapse?

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23. What is the role of oncotic pressure in the capillaries?

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24. Which of the following is true regarding invertebrate circulation?

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25. Which of the following is true about gymnosperms?

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26. Which characteristic is unique to the phylum Mollusca?

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27. What is the primary feature that distinguishes the three domains of life: Archaea, Bacteria, and Eukarya?

28 / 40

28. What is the primary goal of fluorescence recovery after photobleaching (FRAP)?

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29. What type of microscopy is capable of visualizing fluorescent objects and reduces artifacts by focusing a beam of UV light onto the sample?

30 / 40

30. What is a haplotype?

31 / 40

31. What is the role of telomerase in DNA replication?

32 / 40

32. In prokaryotic transcription, how does a termination sequence end the transcription process in Rho independent termination?

33 / 40

33. How many pairs of autosomes are present in the human body?

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34. What is the term for the division of the nucleus?

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35. What is the main difference between heterotrophs and autotrophs?

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36. Where does beta-oxidation of free fatty acids occur in eukaryotic cells?

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37. Which of the following is not evidence supporting the endosymbiotic theory?

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38. Which organelle is responsible for synthesizing lipids, producing steroid hormones, and detoxifying cells?

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39. Which of the following statements correctly describes the role of cholesterol in the cell membrane?

40 / 40

40. Which type of bond is formed between two monosaccharides to form a disaccharide?

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DAT Biology Practice Test #6

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1. In the context of animal behaviors, what is the purpose of communication?

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2. Which type of learning is NOT necessarily associated with reinforcement but involves an animal learning by observing another animal perform the same behavior?

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3. What does the term 'brackish water' refer to in the context of aquatic biomes?

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4. What kind of organism is an autotroph?

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5. How does biochemistry provide evidence of evolution?

6 / 40

6. During oogenesis, when does an ovum complete meiosis I?

7 / 40

7. What is the purpose of positive feedback loops in hormonal regulation?

8 / 40

8. What differentiates the papillary dermis from the reticular dermis?

9 / 40

9. What is the function of the keratinocytes in the epidermis?

10 / 40

10. Which hormone is inhibited by the intake of alcohol, leading to increased urination?

11 / 40

11. What is the primary function of the villi and microvilli in the small intestine?

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12. Where does most digestion and nutrient absorption occur within the human body?

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13. Which gland produces melatonin, and what is its main function?

14 / 40

14. Which hormone stimulates the release of glucocorticoids from the adrenal gland?

15 / 40

15. Which type of joint partially moves and is cartilaginous?

16 / 40

16. Which of the following statements accurately describes the H zone?

17 / 40

17. What is rigor mortis?

18 / 40

18. What is the function of microglial cells?

19 / 40

19. Which of the following is not a part of the neuron?

20 / 40

20. Which type of immunity is gained from receiving antibodies from another organism that already has that immunity?

21 / 40

21. How does decreased pH affect hemoglobin's affinity for oxygen?

22 / 40

22. Which of the following conditions is caused by rapid breathing?

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23. Which of the following statements is true about the chordates' circulatory system?

24 / 40

24. Which of the following statements is true about heart sounds?

25 / 40

25. Which of the following is not a component of the pistil?

26 / 40

26. Which of the following is a characteristic of fungi?

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27. Which phylum is characterized by having a gastrovascular cavity for digestion?

28 / 40

28. What is the difference between totipotent, pluripotent, and multipotent cells?

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29. Which of the following cellular biological lab techniques is based on the principle of electrical resistance?

30 / 40

30. What is a Pedigree chart used for?

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31. Which of the following best describes the role of the TATA box in eukaryotic transcription?

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32. What is the function of topoisomerase in the process of DNA replication?

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33. What is the purpose of anchorage dependence in cell regulation?

34 / 40

34. What happens during the S phase of the cell cycle?

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35. What is the final product of the Calvin cycle?

36 / 40

36. Why are proteins considered the least desirable source of energy?

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37. What happens to pyruvate in pyruvate oxidation?

38 / 40

38. Which of the following statements correctly describes the function of microfilaments in the cytoskeleton?

39 / 40

39. What is the main difference between integral and peripheral membrane proteins?

40 / 40

40. What is a proteome?

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